Solvent system for peroxidase inhibitor reagent for fecal occult blood determinations
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United States of America Patent
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Jan 15, 1985
Grant Date -
N/A
app pub date -
Dec 20, 1982
filing date -
Dec 20, 1982
priority date (Note) -
Expired
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Abstract
A process and reagent for determining the presence of fecal occult blood in a test sample using a guaiac-peroxidase test matrix and a means for inhibiting peroxidase enzyme interferences which combines the partial denaturing of peroxidase enzyme protein using a protein-hydrogen bond cleaving agent, with the removal of metals necessary for efficient peroxidase enzyme activity wherein the improvement comprises dissolving said reagent for cleaving protein-hydrogen bonds in an alcohol-water solvent before adding said reagent to the sample.
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