Labeled anti-hapten antibodies and their use as a universal reagent for solid phase radio- and/or enzyme-immunoassays

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United States of America Patent

PATENT NO 4495296
SERIAL NO

06041127

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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A process for detecting the presence of an antigen in a specimen is described, which process comprises: (A) contacting said specimen with a substrate coated with antibodies of said antigen, incubating the contacted substrate and washing the substrate; (B) contacting the washed material of step (A) with a hapten conjugated antibody against said antigen, incubating the so-contacted material and washing the so-incubated material; (C) contacting the washed material of step (B) with a radioactive material labeled or enzyme containing anti-hapten antibody, incubating the so-contacted material and washing the same; and (D) effecting radioimmunoassay if said antibody is radioactive or enzyme labeled immunoassay if said antibody contains an enzyme moiety. Quantitative determination of the antigen in the specimen is effected by comparing the counts of the radioimmunoassay or the concentration of enzyme against a standard as by photocolormetric methods.

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Patent Owner(s)

  • NEW YORK BLOOD CENTER, INC.;SYMMETRY MEDICAL USA, INC.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Neurath, A Robert New York, NY 7 190
Strick, Nathan Far Rockaway, NY 16 149

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