Method for purifying gamma-interferon
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United States of America Patent
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Feb 12, 1985
Grant Date -
N/A
app pub date -
Feb 7, 1984
filing date -
Feb 7, 1984
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A method is provided for restoring some or all of the activity of gamma-interferon which has been in contact with an acidic solution comprising the steps of: (a) placing the gamma-interferon in a solution which has a pH between about 5.5 and 9.5; and (b) incubating the solution at a temperature of between about 2.degree. C. and 8.degree. C. for a period of at least 24 hours. The method allows antibody affinity chromatography employing acid elution to be used to purify gamma-interferon, in that, the activity of the acid-eluted gamma-interferon can be essentially completely restored using the reactivation process.
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