Bacteria carrying plasmid having temperature dependent plasmid copy number
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United States of America Patent
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Feb 12, 1985
Issued Date -
N/A
app pub date -
Dec 8, 1980
filing date -
Dec 30, 1977
priority date (Note) -
Expired
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Abstract
Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a plasmid which shows a controlled constant plasmid copy number at one temperature and a much higher or totally uncontrolled copy number at a different temperature. The plasmid may be prepared by recombinant DNA technique using a cloning vector showing the temperature dependent plasmid copy number pattern.
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