Biological detection process using polymer-coated electrodes
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United States of America Patent
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May 14, 1985
Grant Date -
N/A
app pub date -
Aug 15, 1983
filing date -
Aug 15, 1983
priority date (Note) -
Expired
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Abstract
A method is described for the amperometric determination of the concentration in a biological sample of a constituent which is a substrate for an oxidase enzyme by (1) catalyzing the substrate with the enzyme, thereby consuming O.sub.2 and producing H.sub.2 O.sub.2 and (2) measuring the O.sub.2 consumption or H.sub.2 O.sub.2 production by means of an inert metal indicator electrode intimately coated with a thin film of a perfluorosulfonic acid polymer. A reaction chamber is described which provides means for contacting the sample with the enzyme and means for making amperometric measurements utilizing inert metal indicator electrodes coated with a thin film of a perfluorosulfonic acid polymer.
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