Colony hybridization method
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United States of America Patent
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-
Aug 6, 1985
Grant Date -
N/A
app pub date -
Aug 3, 1983
filing date -
Aug 3, 1983
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
In the colony hybridization technique, bacterial colonies are replica-plated onto filter paper, and their DNA is hybridized with labeled DNA containing a specific sequence. A one-hundred fold increase in sensitivity is obtained by subjecting the colonies to a stream of steam in the presence of alkali for about three minutes prior to hybridization.
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