Tissue culture medium
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United States of America Patent
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-
Aug 6, 1985
Grant Date -
N/A
app pub date -
Jan 26, 1983
filing date -
Jan 26, 1983
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
A growth factor containing serum is disclosed which is derived from natural bovine serum is disclosed which is by precipitating a first solids fraction from natural bovine serum by adding ammonium sulfate to about 25% saturation to give a first solids fraction and a first supernatant; separating the first solids fraction from the first supernatant; precipitating a second solids fractions from the first supernatant by adding ammonium sulfate to about 40% saturation to give a second solids fraction and a second supernatant; separating the second solids fraction from the second supernatant; combining the first solids fraction and the second supernatant; and desalinating the first solids fraction and the second supernatant, either before or after combination thereof.
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