Assay method and probe for polynucleotide sequences
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United States of America Patent
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Dec 3, 1985
Issued Date -
N/A
app pub date -
Feb 1, 1983
filing date -
Jul 26, 1982
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Abstract
An assay method for the detection of a specific nucleotide target sequence in a polynucleotide test extract is disclosed which utilizes a polynucleotide modified probe including both a cDNA sequence substantially complementary to the specific target sequence and a protein binding sequence. The assay is conducted by exposing the modified probe to the polynucleotide test extract for hybridization and then exposing the complex to the protein which binds to the protein binding sequence. An assay, such as an immunoassay, can then be conducted on the test sample to indicate the presence of the specific target sequence by detecting the presence of the binding protein.
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