Cryopreservation of biological materials in a non-frozen or vitreous state
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United States of America Patent
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Dec 17, 1985
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N/A
app pub date -
Nov 23, 1982
filing date -
Nov 23, 1982
priority date (Note) -
Expired
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Abstract
A method is provided for the successful cryopreservation of biological materials including whole organs, organ sections, tissues and cells, in a non-frozen (vitreous) state, comprising cooling the biological material to be preserved under pressure in the presence of a non-toxic vitrifable protective solution to at least the glass transition temperature thereof to vitrify the solution without substantial nucleation or ice crystal growth and without significant injury to the biomaterial. The invention also provides non-toxic protective vitrification solutions useful in the cryopreservation of biomaterials.
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