Diagnostic test for hepatitis B virus

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United States of America Patent

PATENT NO 4562159
SERIAL NO

06249369

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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A method and test kit are disclosed for detecting the presence of hepatitis B virus in a test specimen containing at least a portion of the DNA of the virus. A test reagent comprises cloned hepatitis B virus-DNA that has been repurified by treatment with a restriction enzyme and labelled to high specific activity with a radioactive label. The sample to be tested is fixed to a solid matrix, incubated in the presence of the test reagent under hybridization conditions and detected by hybridization to the labelled DNA probe. The uncombined HBV-DNA (labelled) is removed from the substrate, and the hybridized HBV-DNA determined by scintillation counting or by autoradiography of the substrate.

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Patent Owner(s)

Patent OwnerAddress
ALBERT EINSTEIN COLLEGE OF MEDICINE 1300 MORRIS PARK AVE BRONX NY 10461 A DIVISION OF YESHIVA UNIVERSITYNY

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Shafritz, David A Larchmont, NY 3 55

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