US Patent No: 4,608,339

Number of patents in Portfolio can not be more than 2000

Protoplast fusion method for high-frequency DNA transfection in human cells

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Abstract

A modified protoplast fusion method and cell line is disclosed that stably transfects human cells with pSV2-derived plasmids at frequencies greater than 10.sup.-3. This procedure makes it possible to test the biological effect of individual genes (i.e., oncogenes and other cellular genes, and viral genes). To demonstrate the utility of this invention, a pSV2gpt.sup.+ plasmid constructed to carry a subgenomic fragment of hepatitis B virus (HBV) that contained the core antigen gene (HBc gene) is transfected into human cells. Human cell lines are stably transfected with the HBC.sup.+ gene by selecting recipient cells for expression of guanine phosphoribosyl transferase expression; other selective markers, i.e., neomycin resistance, can also be used. Conditions for enhancing the expression of the transfected gene(s) have also been developed. For example, with this gpt.sup.+ /HBc.sup.+ cell line it is shown that growth in serum-free medium or treatment with 5'-azacytidine stimulates the production of the HBV core antigen.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Harris, Curtis C Garrett Park, MD 82 269
Korba, Brent E Laurel, MD 9 35
Lechner, John F Albuquerque, NM 10 145
Yoakum, George H 20304 Sandsfield Ter., Germantown, MD 20874 2 27

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Patent Info (Count) # Cites Year
 
GLAXO GROUP LIMITED (5)
6,077,697 Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes 32 1996
6,025,155 Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes 62 1996
6,743,967 Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes 15 1998
8,389,802 Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes 0 2005
8,288,610 Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes 0 2006
 
BIOLOGICAL RESEARCH CENTER OF THE HUNGARIAN ACADEMY OF SCIENCES, THE (3)
5,288,625 Mammalian artificial chromosomes 40 1991
5,712,134 Method of producing a cell carrying an excess of mammalian centromeres 27 1995
5,891,691 Method of producing a cell carrying an excess of mammalian centromeres and the cell line carrying an excess of mammalian centromeres 23 1996
 
BAYER AKTIENGESELLSCHAFT (1)
5,059,643 Stabilized polyamides 3 1987
 
BECKMAN INSTRUMENTS, INC. (1)
5,168,057 Trifunctional conjugates 36 1991
 
BOEHRINGER INGELHEIM INTERNATIONAL GMBH (1)
5,965,404 Method for introducing nucleic acids into higher eukaryotic cells 6 1997
 
INSTITUT PASTEUR (1)
5,112,748 Hybrid cells producing an antigen characteristic of the hepatitis B virus obtained from hepatocytes and established monkey cells, a process for obtaining these hybrid cells and their application to the production of the aforesaid antigen 0 1987
 
NOVARTIS AG (1)
5,716,827 Human hematopoietic stem cell 29 1995
 
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA (1)
5,989,814 Screening methods in eucaryotic cells 15 1997