Adsorption-desorption purification of glucose isomerase
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United States of America Patent
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Sep 9, 1986
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N/A
app pub date -
Dec 20, 1984
filing date -
Dec 20, 1984
priority date (Note) -
Expired
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Abstract
The present invention relates to a process for the production of a purified glucose isomerase which comprises contacting an impure extract containing glucose isomerase and soluble impurities with a weakly basic ion exchange material known to adsorb glucose isomerase; adding a first salt solution of a concentration which removes unadsorbed and weakly adsorbed impurities, but not glucose isomerase; and adding a second, buffered salt solution which elutes the adsorbed glucose isomerase.
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