Assaying for circulating immune complexes with labeled protein A
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United States of America Patent
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Oct 14, 1986
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N/A
app pub date -
Jul 22, 1983
filing date -
Jul 22, 1983
priority date (Note) -
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Abstract
A method for assaying circulating immune complexes comprises contacting the circulating immune complexes in solution in serum with a staphylococcal protein-A linked to a detectable label, whereby a CIC-protein-A-label complex is formed, selectively precipitating the CIC-SPA-label complex by contacting the complex with polyethylene glycol, separating the precipitated CIC-SPA-label complex from the serum, measuring the quantity of the label in the precipitated CIC-protein-A-label complex comparing the measured quantity of label with at least one standard prepared by subjecting a solution containing a known amount of CIC or functional equivalent material to the same assay. The method requires only a single precipitation step and in a preferred embodiment the formation of the CIC-SPA-label complex may be formed in a single step.
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