Process and composition for the rapid quantitation of small levels of creative kinase-MB isoenzyme
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United States of America Patent
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Nov 25, 1986
Grant Date -
N/A
app pub date -
Apr 6, 1984
filing date -
Apr 6, 1984
priority date (Note) -
Expired
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Abstract
A process for conducting fast, rapid two site, two step immunoradiometric tests for CK-MB. In a first series of steps, a mobile particulate solid-phase having an isoenzyme selected from the group of CK-BB and CK-MM immobilying bound on its surface is suspended in a matrix of pooled match human serum. The mixture is incubated, diluted, and centrifuged. In a second series of steps, the resulting solid is incubated with a radio labeled antibody that is specific to the isoenzyme not used in the first series of steps. Counting the radioactivity gives the amount of CK-MB in the patients serum.
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