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Abstract

An improved agitator for use with a fermentation and tissue culturing vessel acting as a microcarrier culture vessel is provided. The agitator includes a hollow body assembly having an open end and a closed end and at least one tubulation communicating with the hollow body assembly at a location spaced from the open end thereof, the tubulation having an exit opening oriented such that movement of the agitator and the tubulation causes a suction or draft at the exit opening to cause flow of fluid from the open end of the hollow body assembly through the hollow body assembly and out through the tubulation exit opening. In this way, a microcarrier cell culture may be stirred in a smooth and non-erratic way thereby minimizing the detachment of the rounded growth cells from the microcarriers.

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