Process for extracting atrial natriuretic factor
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United States of America Patent
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Mar 3, 1987
Issued Date -
N/A
app pub date -
Dec 18, 1984
filing date -
Feb 22, 1982
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Expired
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Abstract
Diuretic and natriuretic extracts, at least partially characterized as peptides, have been obtained by homogenization of mammalian heart atria with an aqueous solution of a lower carboxylic acid. After precipitation of impurities by pH adjustment, the extract may be further purified chromatographically. Extracts injected into text rats resulted in 30-40 fold increases in sodium and chloride excretions within 5-10 minutes of injection. Urine volume rose 10-15 fold and potassium excretion doubled. The response was complete in 20 minutes and no similar changes in renal function were observed following injection of a similarly obtained ventricular extract.
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