Hybridization method of detecting nucleic acid sequences with probe containing thionucleotide
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United States of America Patent
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Mar 3, 1987
Grant Date -
N/A
app pub date -
Jun 1, 1984
filing date -
Jun 1, 1984
priority date (Note) -
Expired
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Abstract
Improved hybridization probe compositions and method for detecting nucleic acids are provided. A hybridization probe constructed to contain thionucleotides is hybridized to a nucleotide sequence of interest in the absence of formamide, DTT and dextran sulfate. If a cold (.sup.32 S) thionucleotide is incorporated into the probe molecule, the probe is provided with a detectable label, radioactive or otherwise.
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