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Abstract

Methods for the detection and identification of unknown pathogens or genetic entities from samples suspected of containing the same involve the use of novel wicking techniques whereby sample DNA/RNA becomes readily available on a porous, inert, positively charged support in single stranded form for hybridization with a hybridization probe having a nucleotide sequence substantially complementary to a nucleotide sequence of the sample DNA/RNA. Such methods permit the detection and identification of unknown pathogens and genetic entities in a shorter period of time than that required by conventional methods. Lysis solutions for rapidly lysing bacterial cells without the aid of enzymes are also disclosed.

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