Gene amplification in eukaryotic cells
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United States of America Patent
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Apr 7, 1987
Issued Date -
N/A
app pub date -
Apr 12, 1985
filing date -
Jan 11, 1982
priority date (Note) -
Expired
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Abstract
Method and compositions are provided for gene amplification. A DNA unit is prepared capable of replication in a eukaryotic host having tandem genes, where the first gene is capable of complementing an auxotrophic host. The auxotrophic host is transformed with the DNA unit under selective conditions requiring enhanced expression of the first gene. Hosts are then selected for amplification of the first and second genes.
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