Method for producing intact plants containing foreign DNA
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United States of America Patent
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Apr 14, 1987
Grant Date -
N/A
app pub date -
Jul 25, 1984
filing date -
Jul 25, 1984
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The invention discloses an in vivo method for transforming and regenerating intact plants. According to the method, a plant (P) is infected with an infectious microbial agency comprised of: (1) virulence functions, (2) oncogenic factors capable of inducing a shoot-bearing shooty tumor on plant (P), and (3) a carrier vector containing engineered heterologous transfer DNA capable of being integrated into the nuclear DNA of plant (P) cells. Infected plant (P) is maintained until a shoot-bearing shooty tumor develops at or near the infection site. Those shoots, or progeny thereof, that contain transformed cells having heterologous transfer DNA integrated into their genomes are the selected and utilized to produce whole plants that contain cells having heterologous transfer DNA integrated into their genomes.
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