Producing lipid-protein membranes for chemical detection
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United States of America Patent
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Apr 28, 1987
Grant Date -
N/A
app pub date -
Jun 20, 1984
filing date -
Jun 20, 1984
priority date (Note) -
Expired
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Abstract
Stable lipid-protein membranes are prepared by providing a medium of dissolved and emulsified lipids and dissolved proteins. The medium is applied to an orifice formed in a sheet of material in sufficient volume to provide sufficient lipid and protein to form a membrane across the orifice, which membrane comprises a bimolecular lipid layer with protein molecules interspersed therein. The medium is allowed to dry in the presence of air, and the lipid and protein molecules arrange themselves to form the membrane. The protein is selected to be specifically reactive with a particular chemical substance or a narrow class of chemical substances. When the membrane is exposed to a substance with which the membrane proteins react, a physical characteristic of the membranes is altered. Detection of a change in such membrane characteristic confirms the presence of the substance.
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