Assays utilizing labeled nucleic acid probes
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United States of America Patent
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Jun 2, 1987
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N/A
app pub date -
May 23, 1984
filing date -
May 23, 1984
priority date (Note) -
Expired
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Abstract
In a method for determining a particular polynucleotide sequence in a test medium containing single stranded nucleic acids wherein the sample is subjected to a hybridization reaction with a labeled detection probe having a substantially complementary polynucleotide sequence, and wherein after hybridization the label in said probe is assayed, the improvement wherein the label in said labeled probe comprises a fluorescent nucleotide which is linked by a phosphate ester linkage to said probe. Probes and kits therefor are also provided.
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