Apparatus and method for cryopreparing biological tissue
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United States of America Patent
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Jun 30, 1987
Issued Date -
N/A
app pub date -
Aug 29, 1985
filing date -
Nov 30, 1984
priority date (Note) -
Expired
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Abstract
This invention relates to method and apparatus for the cryopreparation of biological tissue samples for ultrastructural analysis. The use of the apparatus comprises vitrifying a biological tissue sample under cryogenic temperature conditions and ultra low vacuum conditions. The depressurized, vitrified tissue sample is brought to equilibrium in a sample holder at a temperature of less than -140.degree. C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is optionally infiltrated with a degassed resin followed by a polymerization of the resin to form an embedded tissue sample.
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