Radioactive labeling of proteins with nucleosides or nucleotides
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United States of America Patent
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Sep 8, 1987
Grant Date -
N/A
app pub date -
Nov 27, 1984
filing date -
Nov 27, 1984
priority date (Note) -
Expired
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Abstract
A radioactively labeled protein comprising a protein, and a radioactive nucleoside or nucleotide, the protein being covalently linked to the nucleoside or nucleotide. Advantageously the linkage is through an NH.sub.2 group of the protein and through a carbonyl group of a ring-opened sugar moiety of the nucleoside or nucleotide. The protein can be insulin, an immunoglobulin or protein A. The radioactive moiety may be a P, C, S, H, I or Hg atom. The labels can be used to indicate the presence and amount of the protein in a biological assay.
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