Methods for protein binding enzyme complementation assays
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United States of America Patent
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Nov 24, 1987
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N/A
app pub date -
Apr 8, 1985
filing date -
Oct 29, 1984
priority date (Note) -
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Abstract
This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristic of .beta.-galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.
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