Portable temperature-sensitive control cassette
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United States of America Patent
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-
Dec 8, 1987
Issued Date -
N/A
app pub date -
Dec 24, 1984
filing date -
Aug 31, 1984
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
Two types of convenient portable control cassettes for the expression of protein encoding sequences in procaryotes are disclosed. Both cassettes comprise the P.sub.L promoter from lambda phage, which is controllable by means of a temperature sensitive repressor, operably linked to the ribosome binding site for N-gene (N.sub.RBS). In one embodiment, this cassette is bordered by restriction sites upstream of the P.sub.L promoter and immediately downstream from the N.sub.RBS permitting the insertion of a desired sequence containing its own start codon downstream from the cassette. The other embodiment contains an ATG start codon within the cassette and has a restriction site immediately 3' of the start codon.
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