Method and apparatus for distinguishing multiple subpopulations of cells
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United States of America Patent
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Jan 5, 1988
Issued Date -
N/A
app pub date -
Oct 15, 1984
filing date -
Aug 30, 1982
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Abstract
A method of distinguishing multiple subpopulations of cells from a single sample of cells of a variety of types comprises labeling particles with two or more marking agents. These particles are marked in a plurality of different pre-selected ratios of the agents ranging between zero percent and one hundred percent of each agent. Each such agent has distinguishing, quantifiable marking characteristics. The differently labeled particles are mixed with cells suspected of having specific receptors for the differently labeled particles. Each cell is analyzed to determine the ratio of any two identifiable marking characteristics associated with each cell so that is can be classified in a subpopulation category if its ratio of marking characteristics is related to one of the pre-selected ratios of marking agents. An apparatus for carrying out the above-described method is also within the purview of the present invention.
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