Reagent polynucleotide complex with multiple target binding regions, and kit and methods
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United States of America Patent
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Feb 16, 1988
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N/A
app pub date -
Sep 19, 1985
filing date -
Sep 19, 1985
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Expired
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Abstract
Reagent complexes containing a probe polynucleotide with at least two target binding regions. A labeled polynucleotide is bound to at least a portion of each target binding region. In one method, sample nucleic acid displaces labeled polynucleotide from one target binding region; after washing, a reagent polynucleotide displaces labeled polynucleotide from the other target binding region. In a second method, sample nucleic acid strands having two target nucleic acid sequences in proximity (e.g., sequences translocated in certain cancers) displace the labeled polynucleotide from both target binding region.
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