Displacement polynucleotide method and reagent complex employing labeled probe polynucleotide

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United States of America Patent

PATENT NO 4752566
SERIAL NO

06809992

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Abstract

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A method for determining the presence and amount of a predetermined target nucleotide sequence in a biological sample is provided. The method employs a reagent complex which consists of a labeled probe polynucleotide having a target binding region and a second polynucleotide bound to the labeled probe in a second polynucleotide binding region thereof. The second binding region is at least partially coextensive with the target binding region. This reagent complex is contacted with a sample under conditions in which the target sequence binds to the labeled probe, displacing the second polynucleotide. Labeled probe/target hybrids are separated from intact reagent complexes and the presence and amount of these hybrids are determined.

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Patent Owner(s)

Patent OwnerAddress
GENETICS INSTITUTE LLC87 CAMBRIDGE PARK DRIVE CAMBRIDGE MASSACHUSETTS 02140 UNITED STATES OF AMERICA

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Collins, Mary Natick, MA 65 2336
Dougherty, Joseph P Somerville, MA 22 928

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