Purification of monomeric interferon
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United States of America Patent
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Aug 23, 1988
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N/A
app pub date -
Oct 6, 1987
filing date -
Oct 6, 1987
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Expired
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Abstract
Fast moving monomers of alpha-2 interferon ('IFN-alpha-2') are purified from slow moving monomers and oligomers of interferon and from noninterferon proteins using a combination of immunoaffinity chromatography, reverse phase HPLC, cation exchange chromatography, and gel filtration. Two fast monomer forms, FMM.sub.1 and FMM.sub.2, are further distinguished on the basis of charge and hydrophobicity and FMM.sub.2 is obtained in essentially homogeneous form with respect to size, hydrophobicity and charge.
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