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Abstract

Fluorogenic compounds are disclosed which are reactive with biologically important analytes including primary amines, secondary amines, and/or thiols and are of the general formula: ##STR1## wherein X is a group such as ##STR2## --CN, --CO.sub.2 CH.sub.3, or SO.sub.2 R, and wherein R is OR.sub.3, or Cl; R.sub.1 and R.sub.2 are hydrogen atoms or alkyl groups having 1 to 3 carbon atoms and R.sub.3 is a phenyl or benzyl group. The inventive compounds form a fluorescent adduct with the above analytes at the number 7 position of the benzoxadiazole ring. The fluorescent adduct thereby formed enables detection of very minute amounts of analyte (picomoles) using conventional fluorescent analytical methods.

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First Claim