Nucleic acid probe detectable by specific nucleic acid binding protein
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United States of America Patent
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Oct 11, 1988
Issued Date -
N/A
app pub date -
Oct 19, 1984
filing date -
Dec 12, 1983
priority date (Note) -
Expired
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Abstract
A nucleic acid detection probe comprising a hybridizable single stranded portion of nucleic acid connected with a non-hybridizable, single or double stranded nucleic acid portion, the non-hybridizable portion preferably including a recognition site for binding by a particular protein. Such recognition site can be a region of singly or doubly stranded nucleic acid specific for a particular nucleic acid binding protein such as lac repressor protein or can be a modified nucleic acid region such as a unique antigenic determinant introduced by interaction of the region with a modifier compound such as an intercalating agent or a platinum-containing ligand. The probe-binding protein can be labeled for ease of detection and in the case of an antigenic determinant binding site can be labeled antibody.
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