High-concentration liposome processing method
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United States of America Patent
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Nov 1, 1988
Grant Date -
N/A
app pub date -
Sep 18, 1986
filing date -
Sep 18, 1986
priority date (Note) -
Expired
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Abstract
A method of preparing a concentrated liposome suspension having a lipid concentration of greater than about 250 .mu.m/ml and liposome sizes no greater than about 0.4 microns. A solution of vesicle-forming lipids in a chlorofluorocarbon solvent is injected under selected conditions into an aqueous medium, with continual solvent removal. During the lipid injection and solvent-removal steps, the liposomes formed in the aqueous medium are extruded through a membrane, to reduce liposome sizes to less than about 0.6 microns. The lipid injection, solvent removal, and extrusion steps are continued until a lipid concentration of at least about 150 .mu.m/ml is reached.
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