Mixture of amino acid derivatives, process of producing the mixture and use of the mixture for quantitative determination of the amino acids
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United States of America Patent
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Nov 15, 1988
Grant Date -
N/A
app pub date -
Feb 25, 1987
filing date -
Feb 26, 1986
priority date (Note) -
Expired
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Abstract
A mixture of fluorescently-excitable amino acid derivatives suitable for use in quantitative amino acid analysis is provided in which primary amino acids are present as derivatives of a first reagent and secondary amino acids are present as derivatives of a second different reagent. The process for preparing the mixture comprises the steps of derivatization of the primary amino acids of an amino acid sample with ortho-phthalaldehyde (OPA) and of derivatization of the secondary amino acids of the sample with fluorenylmethylchloroformate (FMOC) in the presence of acetonitrile. The mixture can be obtained automatically and is highly suitable for fast analysis by reversed-phase liquid chromatography.
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