Nucleotide hybridization assay for protozoan parasites

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United States of America Patent

PATENT NO 4801530
SERIAL NO

07038959

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The present invention provides a method for detection of protozoan parasites in blood or other specimen from their mammalian hosts. The method comprises nucleic acid hybridization of repetitive nuclear DNA fragments of the parasites. Hybridization probes have been prepared for this purpose by cloning the repetitive nuclear elements that are species-specific in appropriate vectors. The sensitivity of these probes has been increased by further sub-cloning to make them capable of cascade hybridization. The assay is highly specific and sensitive for detection of disease-causing protozoan parasites of the commonly occurring Trypanosomatidae of the genus Leishmania and genus Trypanosoma, as well as for malaria-causing protozoan and other parasitic microorganims of mammals.

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Patent Owner(s)

Patent OwnerAddress
ROCKEFELLER UNIVERSITYNEW YORK NY

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Lizardi, Paul New York, NY 5 100
Nogueira, Nadia Princeton, NJ 3 21

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