Hollow fiber cell culture device and method of operation
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United States of America Patent
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Feb 14, 1989
Issued Date -
N/A
app pub date -
Aug 19, 1987
filing date -
Oct 9, 1984
priority date (Note) -
Expired
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Abstract
An improved cell culturing device includes a hollow fiber cartridge having a shell and a plurality of capillaries extending through the shell with at least some of the capillaries having selectively permeable walls. A cell culturing space is located between the shell and capillaries. The improvement includes a chamber containing a second medium supply fluidly connected to the cell culturing space. A pressurizing system pressurizes the medium within the chamber to a level higher than the level of medium flowing through the lumen of the capillaries. A valving mechanism alternatively and selectively restricts flow of medium between the chamber and the cell culturing space through first and second conduits such that circulation is effected in the cell culturing space.
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