Reversible agglutination mediators for separating cells from whole blood

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 4812401
SERIAL NO

07051978

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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Compounds and methods are disclosed for reversibly aggregating particles suspended in a liquid medium. The method comprises combining the liquid medium containing the particles with a polyionic polymer capable of aggregating the particles under conditions suitable for such aggregation. Thereafter, the particles are contacted with a chemical reagent capable of cleaving the polyionic polymer under conditions sufficient to reverse the aggregation. Optionally, magnetic particles are added to the liquid medium in the present method under conditions for non-specific binding and the medium including the aggregates is subjected to a magnetic field gradient to separate the aggregates from the medium. The compounds of the present invention are polyions. The aggregation of the particles is reversible upon contact with chemical agents which cleave at least some of the bonds within the polyionic polymer.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
DADE BEHRING MARBURG GMBH35001 MARBURG41

International Classification(s)

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Lin, Cheng-I San Jose, CA 35 301
Tarnowski, Thomas L South San Francisco, CA 12 154
Ullman, Edwin F Atherton, CA 192 7437

Cited Art Landscape

Patent Info (Count) # Cites Year
 
THE UNIVERSITY OF BRITISH COLUMBIA (1)
* 4452773 Magnetic iron-dextran microspheres 338 1982
 
CALIFORNIA INSTITUTE OF TECHNOLOGY (1)
* 4369226 Polyglutaraldehyde synthesis and protein bonding substrates 28 1981
 
SCHRODER, CITIZENS OF SWEDEN (1)
* 4687748 Magnetic carbohydrate particles as carriers for affinity separation purposes 17 1983
* Cited By Examiner

Patent Citation Ranking

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Patent Info (Count) # Cites Year
 
Other [Check patent profile for assignment information] (1)
6689615 Methods and devices for processing blood samples 41 2000
 
VERIDEX, LLC (1)
* 2003/0203,507 Increased separation efficiency via controlled aggregation of magnetic nanoparticles 21 2003
 
UNIVERSITY OF UTAH (2)
8034555 Particle analysis assay for biomolecular quantification 0 2006
* 2006/0127,942 Particle analysis assay for biomolecular quantification 6 2006
 
JANSSEN DIAGNOSTICS, LLC (2)
6623982 Increased separation efficiency via controlled aggregation of magnetic nanoparticles 45 1999
* 6620627 Increased separation efficiency via controlled aggregation of magnetic nanoparticles 36 2000
 
HEALTH RESEARCH, INC. (1)
* 5985658 Calmodulin-based cell separation technique 5 1997
 
ABBOTT LABORATORIES (1)
* 5411894 Method of using tissue and cell adhesive preparations for biological test systems 8 1990
 
HOLGER KIESEWETTER (1)
* 2010/0009,383 Method for the simple and rapid detection of cells and biomolecules by means of paramagnetic particles 0 2005
 
CANON KABUSHIKI KAISHA (2)
* 7354995 Magnetic substance-biosubstance complex structure, peptide fragment capable of linking to magnetic substance and gene therefor, and process for producing the complex structure 9 2004
* 2007/0054,315 Magnetic substance-biosubstance complex structure, peptide fragment capable of linking to magnetic substance and gene therefor, and process for producing the complex structure 0 2004
* Cited By Examiner