US Patent No: 4,812,401

Number of patents in Portfolio can not be more than 2000

Reversible agglutination mediators for separating cells from whole blood

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Abstract

Compounds and methods are disclosed for reversibly aggregating particles suspended in a liquid medium. The method comprises combining the liquid medium containing the particles with a polyionic polymer capable of aggregating the particles under conditions suitable for such aggregation. Thereafter, the particles are contacted with a chemical reagent capable of cleaving the polyionic polymer under conditions sufficient to reverse the aggregation. Optionally, magnetic particles are added to the liquid medium in the present method under conditions for non-specific binding and the medium including the aggregates is subjected to a magnetic field gradient to separate the aggregates from the medium. The compounds of the present invention are polyions. The aggregation of the particles is reversible upon contact with chemical agents which cleave at least some of the bonds within the polyionic polymer.

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First Claim

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Patent Owner(s)

Patent OwnerAddressTotal Patents
DADE BEHRING MARBURG GMBH35001 MARBURG403

International Classification(s)

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  • [Patents Count]

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Lin, Cheng-I Cupertino, CA 37 205
Tarnowski, Thomas L San Francisco, CA 12 118
Ullman, Edwin F Atherton, CA 194 5906

Cited Art

Patent Info (Count) # Cites Year
 
CALIFORNIA INSTITUTE OF TECHNOLOGY (1)
4,369,226 Polyglutaraldehyde synthesis and protein bonding substrates 27 1981
 
KENDREW BIOSYSTEMS, INC., A CORP. OF DE (1)
4,230,685 Method of magnetic separation of cells and the like, and microspheres for use therein 112 1979
 
SCHRODER, CITIZENS OF SWEDEN (1)
4,687,748 Magnetic carbohydrate particles as carriers for affinity separation purposes 15 1983
 
THE UNIVERSITY OF BRITISH COLUMBIA (1)
4,452,773 Magnetic iron-dextran microspheres 278 1982

Patent Citation Ranking

Forward Cites

Patent Info (Count) # Cites Year
 
VERIDEX, LLC (2)
6,623,982 Increased separation efficiency via controlled aggregation of magnetic nanoparticles 18 1999
6,620,627 Increased separation efficiency via controlled aggregation of magnetic nanoparticles 13 2000
 
ABBOTT LABORATORIES (1)
5,411,894 Method of using tissue and cell adhesive preparations for biological test systems 8 1990
 
CANON KABUSHIKI KAISHA (1)
7,354,995 Magnetic substance-biosubstance complex structure, peptide fragment capable of linking to magnetic substance and gene therefor, and process for producing the complex structure 6 2004
 
HEALTH RESEARCH, INC. (1)
5,985,658 Calmodulin-based cell separation technique 2 1997
 
UNIVERSITY OF UTAH (1)
8,034,555 Particle analysis assay for biomolecular quantification 0 2006
 
Other [Check patent profile for assignment information] (1)
6,689,615 Methods and devices for processing blood samples 14 2000