Separation of lymphocytes and monocytes from blood samples
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United States of America Patent
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Mar 28, 1989
Issued Date -
N/A
app pub date -
Oct 28, 1986
filing date -
Dec 24, 1984
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Abstract
This invention is directed to means for inhibiting the apparent shift in the buoyant density of and/or to restore any loss in the buoyant density of the granulocytic white blood cells in a sample of blood, thereby insuring the quality of the separation of lymphocytes and monocytes from granulocytes in a blood sample. The invention comprises contacting a blood sample with a hypertonic fluid containing a low molecular weight organic or inorganic ionic substance and/or with an isotonic or hypertonic fluid containing a high molecular weight organic substance which may contain organic molecules having a lipophilic substituent in their structures and/or with a culture medium for blood cells. An integral part of the invention is an improved blood separation tube utilizing a gel-like substance having a specific gravity between 1.060-1.065 g/cm.sup.3 to significantly enhance the purity of cell separation, while providing acceptable cell yields.
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