Fluorescence ligand binding assay using charge-matched dye and solvent components

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United States of America Patent

PATENT NO 4816419
SERIAL NO

06628715

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Abstract

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Fluorescence ligand binding assay of a sample containing an unknown amount of ligand is performed by making direct intensity measurements. In an immunoassay the sample is in a solution containing dye labeled analyte and an antibody specific to the analyte. Surfactant, added to the solution in an amount sufficient to form micelles, provides markedly different fluorescent intensity from bound and unbound labeled analyte if the surfactant ions and dye ions have the same charge polarity and the analyte moeity has the opposite charge polarity.

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Patent Owner(s)

Patent OwnerAddress
HERMAN M FINCH UNIVERSITY OF HEALTH SCIENCES/THE CHICAGO MEDICAL SCHOOL3333 GREEN BAY ROAD NORTH CHICAGO IL 60064-3095

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Halfman, Clarke J Highland Park, IL 2 24

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