Blood partitioning method
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United States of America Patent
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-
Apr 4, 1989
Issued Date -
N/A
app pub date -
Feb 3, 1988
filing date -
Sep 24, 1984
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention is directed to a method for separating mononuclear cells from other cells in a blood sample which is more rapid than the currently used density gradient material and which provides a better separation than methods utilizing thixotropic, gel-like material. In the method, a water soluble density gradient material is placed in a centrifuged tube. A water insoluble, thixotropic, gel-like substance is also placed in the container. A blood sample is placed in the container and the container is centrifuged for a time during which various components interchange positions and the gel-like substance forms a barrier between the mononuclear cells and the other cells of the blood sample.
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