Luminometer apparatus
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United States of America Patent
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-
Apr 4, 1989
Grant Date -
N/A
app pub date -
Dec 16, 1987
filing date -
Dec 17, 1986
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
A luminometer apparatus has a light-tight box (12) into which a cuvette (2) containing a sample and a reagent are located for producing a phosphorescent reaction. The phosphorescent reaction is detected by a photodetector (PD) and a shutter (3) is interposed between the cuvette and the shutter. Output signals from the photodetector are applied to a circuit arrangement (FIG. 4) which detects the phosphorescent reaction at predetermined intervals and by subtracting the successive values of light from one another so a measured peak value of light intensity is determined. The shutter is closed and the dark current signal of the photodetector is determined and subtracted from the measured peak value. A true peak intensity of the emitted light is thus derived and the concentration of the material being assayed is calculated by raising the peak intensity to the power n where n has a value between 0 and 9 predeterminedly derived for the sample.
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