Apparatus for immunohistochemical staining and method of rinsing a plurality of slides

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United States of America Patent

PATENT NO 4847208
SERIAL NO

07079476

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Abstract

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A device and method for automating immunohistochemical staining of biological material on glass slides is presented. The slides are placed on a base with the tissue section facing upwards. A 'chamber' consisting of a rectangular plastic box with a hinged overhead door is clamped directly over the tissue section. A rubber gasket seals the chamber--slide interface. Reagents are dropped on to the tissue section by opening the hinged overhead plastic door. When the door is closed, the chamber is sufficiently sealed so as to prevent evaporation. Washing of the tissue section is accomplished by the addition and aspiration of buffer through ports in the chamber. The ports are connected via flexible tubing to a manifold. Because the wash steps are peformed in tandem and do not require manual manipulation of slides, a significant time savings is realized.

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Patent Owner(s)

Patent OwnerAddress
DAKO DENMARK A/SPRODUKTIONSVEJ 42 GLOSTRUP D-2600

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bogen, Steven A 73 Hampstead Rd., Boston, MA 02130 25 1493

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