Microbiological production of essential fatty acids
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United States of America Patent
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Jul 25, 1989
Grant Date -
N/A
app pub date -
Feb 19, 1985
filing date -
Feb 21, 1984
priority date (Note) -
Expired
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Abstract
Production of .gamma.-linolenic acid, by growth of a diffused mycelial inoculum of Rhizopus arrhizus in a stirred, aerated nutrient medium containing a carbohydrate energy source and an inorganic nitrogen source at a carbon:nitrogen ratio of 20:1 to 60:1 maintained at a temperature of 25.degree. C..+-.2.degree. C. and a pH of 3.75 to 6.25 in the presence of a foam breaker, until a .gamma.-linolenic concentration of at least 1.0 g/liter of medium is reached, harvesting the organism, and extraction of lipid containing the .gamma.-linolenic acid.
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