Method of compensating and calibrating a flow cytometer, and microbead standards kit therefor
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United States of America Patent
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-
Aug 15, 1989
Issued Date -
N/A
app pub date -
Dec 4, 1987
filing date -
Dec 24, 1984
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
A kit of highly uniform microbead standards for flow cytometer alignment, compensation, and calibration, comprising a blank and/or auto-fluorescent microbead population, together with two or more series of calibrated microbead populations which match the fluorescence spectra of labeled samples to be measured on the flow cytometer. Also disclosed is a corresponding method to align, compensate, and calibrate a flow cytometer so as to make measurements on corresponding samples comparable and independent of the specific instrument and instrument settings.
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