Cell growth rate determination by measurement of changes in cyanine dye levels in plasma membranes
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United States of America Patent
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Aug 22, 1989
Grant Date -
N/A
app pub date -
Oct 31, 1986
filing date -
Oct 31, 1986
priority date (Note) -
Expired
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Abstract
Methods for determining growth rate of cells growing in vivo and in vitro. Cells are labelled with cyanine dyes and changes in plasma membrane cyanine dye levels are used to determine growth rate. Cell growth rate determinations are utilized to monitor transplanted bone marrow cell engraftment and post-surgical corneal epitheal cell growth. The invented methods also are used to determine tumor cell sensitivity to cancer therapeutic agents, yeast sensitivity to antifungal agents, and bacteria sensitivity to antibacterial agents.
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