In-vitro methods for identifying compositions which are agonists and antagonists of estrogens
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United States of America Patent
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Aug 22, 1989
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N/A
app pub date -
Apr 17, 1986
filing date -
Apr 17, 1986
priority date (Note) -
Expired
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Abstract
The present invention provides two general protocols by which a substance may be identified and characterized as an estrogen agonist and/or an estrogen antagonist. The protocols are in-vitro methods which utilize estrogen dependent cells in culture and a medium comprising an inhibitor endogenous to the sera of adult humans which is able to prevent the proliferation of cultured cells in-vitro. The methodology is rapid, reproducible, accurate and provides the major advantage of being able to screen large numbers of substances for their primary or secondary side effects in a large variety of medical and environmental applications.
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