Method of detecting, isolating and purifying clostridium difficile toxin A and its receptors
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United States of America Patent
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Sep 5, 1989
Issued Date -
N/A
app pub date -
Feb 11, 1986
filing date -
Jul 3, 1985
priority date (Note) -
Expired
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Abstract
A method is provided for detecting the presence of C. difficile toxin A. Stool or other appropriate specimen is contacted with a reagent containing an available non-reducing galactose-alpha-1-3-galactosyl structure, which is a specific receptor for toxin A. The reagent may be intact cells, cell membranes, membrane fractions containing the toxin A receptor structure, glycoconjugates, as well as the purified toxin A receptor per se. Binding of toxin A is determined by conventional assay techniques. The method may also be used to isolate and purify toxin A. Conversely, immobilized toxin A is used to detect, isolate, or purify biological materials of interest containing the receptor structure.
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