US Patent No: 4,867,887
Number of patents in Portfolio can not be more than 2000
Method and apparatus for separating mononuclear cells from blood
Stats
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Sep 19, 1989
Issued date -
Jul 12, 1988
filing date -
07/217,947
serial no -
Expired
status
Importance
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Abstract
A blood separation device includes a collection tube containing a first layer of a thixotropic gel-like substance and a second layer of a newtonian gel-like substance which is positioned below the first layer. The first layer of the thixotropic substance is positioned between the second layer of the newtonian substance and a blood sample received by the tube, prior to centrifuging the tube, and acts as a barrier between the second layer and the blood sample to prevent intermixing of the second layer and the blood sample. The thixotropic gel-like substance of the first layer has a specific gravity which is greater than the newtonian gel-like substance of the second layer whereupon centrifugation of the tube, the first layer moves to a position below the second layer. The second layer of the newtonian gel-like substance acts as a density separation medium, the newtonian gel-like substance being formed with a specific gravity such that the second layer is adapted to assume a position in the collection tube, upon centrifugation of the tube, between the mononuclear cells and the heavier components of the blood sample placed in the tube.
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First Claim
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International Classification(s)
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Cited Art
| Patent Info | (Count) | # Cites | Year |
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| 4,190,535 Means for separating lymphocytes and monocytes from anticoagulated blood | 61 | 1978 | |
| 4,350,593 Assembly, compositions and method for separating blood | 49 | 1979 | |
| 4,417,981 Blood phase separator device | 77 | 1981 | |
| 4,640,785 Separation of lymphocytes and monocytes from blood samples | 19 | 1984 | |
| 4,816,168 Separation of lymphocytes and monocytes from blood samples | 27 | 1986 | |
| 4,818,418 Blood partitioning method | 43 | 1988 | |
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| 4,435,293 Particle washing system and method of use | 25 | 1981 | |
| 4,436,631 Multiple particle washing system and method of use | 32 | 1982 | |
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| 4,457,782 Composition for partitioning blood components | 20 | 1981 | |
| 4,534,798 Composition for partitioning blood components | 13 | 1983 | |
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| 4,751,001 Blood partitioning apparatus | 34 | 1984 | |
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| 4,487,700 Method and apparatus for separating lymphocytes from anticoagulated blood | 25 | 1983 | |
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| 4,310,430 .alpha.-Olefin-dialkylmaleate-based liquid separating agent | 23 | 1980 | |
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| 4,255,256 Medium for the separation of human blood leucocytes | 28 | 1979 | |