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Abstract

In accordance with the practices of this invention, there is provided a heterologous detection system and components useful in conjunction therewith and kits for carrying out the heterologous detection system. The heterologous detection system employs a heterologous entity and a signalling entity. The heterologous entity contains two free complex forming sites that form different complexes, i.e., two kinds of complexes can be formed. The first free complex forming site is utilized to recognize the labeled probe and the second free complex forming site is utilized to recognize the signalling entity, with each complex formed being different. The signalling entity contains a free complex forming site that can recognize the second free complex forming site of the heterologous entity and a signalling portion that is capable of generating a signal. An example of a heterologous detection system that can be utilized to detect biotinylated probe is a system that contains a heterologous entity comprising avidin and a lectin and a signalling entity comprising a glycosylated enzyme. The avidin can recognize the biotin of the probe and the lectin can recognize the glycosyl group of the enzyme. The enzyme is capable of generating a signal when treated with a suitable reagent. Such a heterologous detection system provides both a quick and highly sensitive technique for detecting a biotinylated probe.

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First Claim