Method for assaying primary amines, secondary amines and peptides using fluorogenic derivatization reagents
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United States of America Patent
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Jan 2, 1990
Grant Date -
N/A
app pub date -
Mar 11, 1987
filing date -
Mar 11, 1987
priority date (Note) -
Expired
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Abstract
Primary amines, secondary amines and peptides are fluorometrically assayed using a fluorogenic derivatization reagent of the formula: ##STR1## where Z is an electron withdrawing group or a fused benzene ring, Y and Y', which are identical or different, are nitrogen or carbon and X is a halogen or other leaving group. The above fluorogenic reagent reacts with the primary amine, secondary amine or peptide to form a cyclic fluorescent adduct.
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